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Generation of mesothelial progenitor‐like cells from mouse‐induced pluripotent stem cells
Author(s) -
Inagaki Natsuko F.,
Inagaki Fuyuki F.,
Kokudo Norihiro,
Miyajima Atsushi
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13325
Subject(s) - induced pluripotent stem cell , stem cell , progenitor cell , microbiology and biotechnology , mesothelial cell , chemistry , biology , embryonic stem cell , pathology , biochemistry , medicine , gene
Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells ( MPC s) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pluripotent stem cells ( iPSC s) have the potential to be used for regenerative medicine. Here, we have established a differentiation protocol for mouse iPSC ‐derived MPC s (mi MPC s) via the exposure to defined factors, as well as purification using MPC ‐specific cell surface antigens. Furthermore, the mi MPC s had the ability to suppress postoperative adhesion and facilitate liver regeneration. This is the first report highlighting the generation of functional mi MPC s, which may offer potential for de novo cell therapy.