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Time‐resolved interaction proteomics of the GIGANTEA protein under diurnal cycles in Arabidopsis
Author(s) -
Krahmer Johanna,
Goralogia Greg S.,
Kubota Akane,
Zardilis Argyris,
Johnson Richard S.,
Song Young Hun,
MacCoss Michael J.,
Le Bihan Thierry,
Halliday Karen J.,
Imaizumi Takato,
Millar Andrew J.
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13311
Subject(s) - arabidopsis , proteomics , biology , circadian clock , gigantea , microbiology and biotechnology , abiotic stress , transcription factor , protein–protein interaction , circadian rhythm , function (biology) , computational biology , biochemistry , botany , mutant , gene , neuroscience
The plant‐specific protein GIGANTEA ( GI ) controls many developmental and physiological processes, mediating rhythmic post‐translational regulation. GI physically binds several proteins implicated in the circadian clock, photoperiodic flowering, and abiotic stress responses. To understand GI 's multifaceted function, we aimed to comprehensively and quantitatively identify potential interactors of GI in a time‐specific manner, using proteomics on Arabidopsis plants expressing epitope‐tagged GI . We detected previously identified (in)direct interactors of GI , as well as proteins implicated in protein folding, or degradation, and a previously uncharacterized transcription factor, CYCLING DOF FACTOR6 ( CDF 6 ). We verified CDF 6's direct interaction with GI , and ZEITLUPE/FLAVIN‐BINDING, KELCH REPEAT, F‐BOX 1/LIGHT KELCH PROTEIN 2 proteins, and demonstrated its involvement in photoperiodic flowering. Extending interaction proteomics to time series provides a data resource of candidate protein targets for GI 's post‐translational control.