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miR‐342‐3p promotes osteogenic differentiation via targeting ATF 3
Author(s) -
Han Yawei,
Zhang Kun,
Hong Yuheng,
Wang Jingzhao,
Liu Qi,
Zhang Zhen,
Xia Han,
Tang Yutao,
Li Tengshuai,
Li Liandong,
Xue Yuan,
Hong Wei
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13282
Subject(s) - osteoblast , gene silencing , microrna , microbiology and biotechnology , runx2 , transcription factor , dna methylation , cpg site , chemistry , ossification , biology , cancer research , gene expression , gene , anatomy , biochemistry , in vitro
Through their multiple targets, micro RNA s (mi RNA s) are involved in numerous physiological and pathological processes. In this study, miR‐342‐3p was found to be deregulated with ossification of ligament or osteoporosis. We demonstrate that silencing miR‐342‐3p impairs osteoblast activity and matrix mineralization, while over expression of miR‐342‐3p promotes osteoblast differentiation significantly. Moreover, miR‐342‐3p directly targets activating transcription factor 3 ( ATF 3), which inhibits transcription of pro‐osteogenic differentiation‐associated genes. In addition, there exists a higher frequency of methylation at the CpG island of the Enah/Vasp‐Like ( EVL ) locus in undifferentiated pre‐osteoblasts; however, demethylation of the EVL CpG island induces over expression of miR‐342‐3p during osteogenic differentiation. This study suggests that miR‐342‐3p may serves as a potential marker for diagnosis and treatment of ossification of ligament and osteoporosis.