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Deadenylation by the CCR 4‐ NOT complex contributes to the turnover of hairy ‐related mRNA s in the zebrafish segmentation clock
Author(s) -
Fujino Yuuri,
Yamada Kazuya,
Sugaya Chihiro,
Ooka Yuko,
Ovara Hiroki,
Ban Hiroyuki,
Akama Kagari,
Otosaka Shiori,
Kinoshita Hirofumi,
Yamasu Kyo,
Mishima Yuichiro,
Kawamura Akinori
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13261
Subject(s) - zebrafish , messenger rna , untranslated region , biology , microbiology and biotechnology , paraxial mesoderm , three prime untranslated region , enhancer , somite , gene , mesoderm , gene expression , genetics , embryo , embryonic stem cell , embryogenesis
In the zebrafish segmentation clock, hairy/enhancer of split ‐related genes her1 , her7 , and hes6 encodes components of core oscillators. Since the expression of cyclic genes proceeds rapidly in the presomitic mesoderm ( PSM ), these hairy ‐related mRNA s are subject to strict post‐transcriptional regulation. In this study, we demonstrate that inhibition of the CCR 4‐ NOT deadenylase complex lengthens poly(A) tails of hairy ‐related mRNA s and increases the amount of these mRNA s, which is accompanied by defective somite segmentation. In transgenic embryos, we show that EGFP mRNA s with 3′ UTR s of hairy ‐related genes exhibit turnover similar to endogenous mRNA s. Our results suggest that turnover rates of her1, her7, and hes6 mRNA s are differently regulated by the CCR 4‐ NOT deadenylase complex possibly through their 3′ UTR s in the zebrafish PSM.