Deadenylation by the  CCR 4‐ NOT  complex contributes to the turnover of  hairy ‐related  mRNA s in the zebrafish segmentation clock | Zendy

Fujino Yuuri | Zendy; Yamada Kazuya | Zendy; Sugaya Chihiro | Zendy; Ooka Yuko | Zendy; Ovara Hiroki | Zendy; Ban Hiroyuki | Zendy; Akama Kagari | Zendy; Otosaka Shiori | Zendy; Kinoshita Hirofumi | Zendy; Yamasu Kyo | Zendy; Mishima Yuichiro | Zendy; Kawamura Akinori | Zendy

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Deadenylation by the CCR 4‐ NOT complex contributes to the turnover of hairy ‐related mRNA s in the zebrafish segmentation clock

Author(s) -

Fujino Yuuri,

Yamada Kazuya,

Sugaya Chihiro,

Ooka Yuko,

Ovara Hiroki,

Ban Hiroyuki,

Akama Kagari,

Otosaka Shiori,

Kinoshita Hirofumi,

Yamasu Kyo,

Mishima Yuichiro,

Kawamura Akinori

Publication year - 2018

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1002/1873-3468.13261

Subject(s) - zebrafish , messenger rna , untranslated region , biology , microbiology and biotechnology , paraxial mesoderm , three prime untranslated region , enhancer , somite , gene , mesoderm , gene expression , genetics , embryo , embryonic stem cell , embryogenesis

In the zebrafish segmentation clock, hairy/enhancer of split ‐related genes her1 , her7 , and hes6 encodes components of core oscillators. Since the expression of cyclic genes proceeds rapidly in the presomitic mesoderm ( PSM ), these hairy ‐related mRNA s are subject to strict post‐transcriptional regulation. In this study, we demonstrate that inhibition of the CCR 4‐ NOT deadenylase complex lengthens poly(A) tails of hairy ‐related mRNA s and increases the amount of these mRNA s, which is accompanied by defective somite segmentation. In transgenic embryos, we show that EGFP mRNA s with 3′ UTR s of hairy ‐related genes exhibit turnover similar to endogenous mRNA s. Our results suggest that turnover rates of her1, her7, and hes6 mRNA s are differently regulated by the CCR 4‐ NOT deadenylase complex possibly through their 3′ UTR s in the zebrafish PSM.

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