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Revealing the metabolic characteristics of human embryonic stem cells by genome‐scale metabolic modeling
Author(s) -
He Yangzhige,
Wang Yan,
Zhang Boya,
Li Yang,
Diao Lihong,
Lu Liang,
Yao Jingwen,
Liu Zhongyang,
Li Dong,
He Fuchu
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13255
Subject(s) - embryonic stem cell , biology , stem cell , computational biology , metabolism , metabolic pathway , metabolic regulation , microbiology and biotechnology , metabolic network , cell metabolism , genetics , gene , biochemistry
Embryonic stem cells ( ESC s) are characterized by a dual capacity, self‐renewal and pluripotency, which can be regulated by metabolism. A better understanding of ESC metabolism and regulatory mechanisms is pivotal for research into development, ageing, and cancer treatment. However, a systematic and comprehensive delineation of human ESC metabolism is still lacking. Here, we reconstructed the first genome‐scale metabolic model ( GEM ) of human ESCs (hESCs). By GEM simulation and analyses, hESC global metabolic characteristics including essential metabolites and network motifs were identified. Potential metabolic subsystems responsible for self‐renewal and pluripotency were also identified by analyses and experiments. This first GEM of hESC s provides a novel view and resource for stem cell metabolism research and will contribute to the elucidation of their metabolic characteristics.