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A kinase‐dead version of FERONIA receptor‐like kinase has dose‐dependent impacts on rosette morphology and RALF 1‐mediated stomatal movements
Author(s) -
Chakravorty David,
Yu Yunqing,
Assmann Sarah M.
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13249
Subject(s) - complementation , rosette (schizont appearance) , kinase , microbiology and biotechnology , protein kinase a , biology , phenotype , chemistry , gene , biochemistry , immunology
The receptor‐like kinase FERONIA ( FER ) pleiotropically affects plant reproduction, development, and stress tolerance. We recently showed that the FER ligand RALF 1 promotes stomatal closure and inhibits stomatal opening in a G‐protein‐dependent manner. FER responses have been designated as kinase‐dependent or kinase‐independent, based largely on fer complementation assays employing a kinase‐dead FERK 565R. Our quantification of FERK 565R‐ GFP transcript and FERK 565R‐ GFP protein in fer complementation lines reveal that, even within individual complementation lines, different levels of FERK 565R expression prevail. FERK 565R‐ GFP expression comparable to that of FER in Col‐0 plants fail to elicit complementation of either fer rosette phenotypes or RALF 1‐elicited stomatal movements, whereas overexpression levels of FERK 565R‐ GFP result in complementation. These results suggest possible alternative interpretations of previous conclusions regarding kinase‐independent FER signaling.