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The Na + ‐coupled glucose transporter SGLT 2 interacts with its accessory unit MAP 17 in vitro and their expressions overlap in the renal proximal tubule
Author(s) -
Calado Joaquim,
Santos Ana Rita,
Aires Inês,
Lebre Firmina,
Nolasco Fernando,
Rueff José,
Ramalho José
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13233
Subject(s) - cotransporter , renal glucose reabsorption , in vitro , chemistry , transporter , glucose transporter , hek 293 cells , reabsorption , kidney , microbiology and biotechnology , biochemistry , biology , receptor , gene , endocrinology , sodium , diabetes mellitus , type 2 diabetes , organic chemistry , insulin
Na + ‐glucose cotransporter 2 is the renal Na + ‐coupled glucose transporter responsible for the tubular glucose reabsorption, while MAP 17 was recently identified as its accessory unit. Mutations in either of the proteins’ coding genes, SLC 5A2 and PDZK 1 IP 1 , lead to urinary glucose excretion. To investigate whether MAP 17 interacts with SGLT 2 in vitro , we engineered a V5‐tagged SGLT 2 construct and evaluated HEK 293T cells coexpressing it together with a HA tagged MAP 17 construct. MAP 17 is shown to colocalize and coimmunoprecipitate with SGLT 2. Also, in human kidney sections, the expression of both proteins overlaps at the apical surface of tubular epithelia. This interaction provides the rationale behind SGLT 2 activation by MAP 17 as well the similarity of the SLC 5A2 and PDZK 1 IP 1 glucosuric phenotypes.