Premium
Nuclear localization of Hif‐3α requires two redundant NLS motifs in its unique C‐terminal region
Author(s) -
Yao Qing,
Zhang Peng,
Lu Ling,
Liu Yunzhang,
Li Yun,
Duan Cunming
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13202
Subject(s) - nls , nuclear localization sequence , nuclear transport , nuclear export signal , nuclear protein , microbiology and biotechnology , biology , nucleus , cell nucleus , gene , genetics , transcription factor
Hif‐3α, a member of the hypoxia‐inducible factor ( HIF ) family, enters the nucleus and regulates gene expression in response to hypoxia. The molecular basis of its nuclear localization is not clear. HIF ‐1α and HIF ‐2α use a bipartite nuclear localization signal ( NLS ) to enter the nucleus. This motif is not conserved in Hif‐3α. Although there is a conserved Arg/Lys rich motif in the Hif‐3α N‐terminal region, deletion of this region has minimal effect on Hif‐3α nuclear localization. Here, we mapped the functional NLS to the unique C‐terminal region of Hif‐3α and identified two clusters of basic residues critical for its nuclear localization. The two NLS motifs are functionally redundant. Our results, thus, suggest that Hif‐3α nuclear localization is mediated through two redundant NLS motifs located in its unique C‐terminal region.