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Linker histone H1 FOO regulates the chromatin structure in mouse zygotes
Author(s) -
Funaya Satoshi,
Ooga Masatoshi,
Suzuki Masataka G.,
Aoki Fugaku
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13175
Subject(s) - chromatin , histone h1 , microbiology and biotechnology , histone , chromatin remodeling , histone h2a , pronucleus , chemistry , histone h3 , solenoid , zygote , biology , embryo , biochemistry , dna , embryogenesis , mechanical engineering , engineering
The chromatin structure in one‐cell‐stage mouse embryos is extremely loose and becomes tighter at the two‐cell stage. As linker histones are involved in higher‐order chromatin structure, we examined the involvement of the linker histone variant H1foo in the change of chromatin looseness between the one‐ and two‐cell stages. H1foo knockdown causes the chromatin structure to be tighter in the pronucleus and increases deposition of the histone H3 variant H3.1/3.2 in the peripheral region of the pronucleus in one‐cell‐stage embryos. The decrease in chromatin looseness at the two‐cell stage is less after overexpressing H1foo. These results suggest that H1foo is involved in the change in chromatin structure via nuclear deposition of H3 variants between the one‐ and two‐cell stages.