Premium
Bdp1 interacts with SNAP c bound to a U6, but not U1, sn RNA gene promoter element to establish a stable protein‐ DNA complex
Author(s) -
Verma Neha,
Hurlburt Ann Marie,
Wolfe Angela,
Kim Mun Kyoung,
Kang Yoon Soon,
Kang Jin Joo,
Stumph William E.
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13169
Subject(s) - small nuclear rna , transcription (linguistics) , rna , promoter , biology , gene , snrnp , genetics , dna , rna polymerase ii , microbiology and biotechnology , transcription preinitiation complex , general transcription factor , non coding rna , gene expression , ribonucleoprotein , linguistics , philosophy
In metazoans, U6 small nuclear RNA (sn RNA ) gene promoters utilize a proximal sequence element ( PSE ) recognized by the small nuclear RNA ‐activating protein complex ( SNAP c). SNAP c interacts with the transcription factor TFIIIB , which consists of the subunits TBP , Brf1 (Brf2 in vertebrates), and Bdp1. Here, we show that, in Drosophila melanogaster , Dm SNAP c directly recruits Bdp1 to the U6 promoter, and we identify an 87‐residue region of Bdp1 involved in this interaction. Importantly, Bdp1 recruitment requires that Dm SNAP c be bound to a U6 PSE rather than a U1 PSE . This is consistent with the concept that Dm SNAP c adopts different conformations on U6 and U1 PSE s, which lead to the subsequent recruitment of distinct general transcription factors and RNA polymerases for U6 and U1 gene transcription.