Premium
Hansenula polymorpha Aat2p is targeted to peroxisomes via a novel Pex20p‐dependent pathway
Author(s) -
Thomas Ann S.,
Krikken Arjen M.,
Boer Rinse,
Williams Chris
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13168
Subject(s) - peroxisome , peroxisomal targeting signal , saccharomyces cerevisiae , cytosol , microbody , biochemistry , yeast , microbiology and biotechnology , biology , protein targeting , receptor , enzyme , membrane protein , membrane
Saccharomyces cerevisiae Aat2p contains a peroxisomal targeting signal type‐1 and localizes to peroxisomes in oleate‐grown cells, but not in glucose‐grown cells. Here, we have investigated Aat2p from the yeast Hansenula polymorpha , which lacks a recognizable peroxisomal targeting signal. Aat2p tagged with GFP at its C terminus displays a dual cytosol‐peroxisome localization in ethanol‐grown cells. The partial peroxisomal localization of Aat2p persisted in the absence of the classical cycling receptors Pex5p and Pex7p but Aat2p targeting to peroxisomes was reduced in cells deleted for the matrix protein import factors PEX 1, PEX 2 and PEX 13. Furthermore, we demonstrate that Aat2p targeting to peroxisomes requires Pex20p. Together, our data identify a Pex20p‐dependent pathway for targeting Aat2p to peroxisomes.