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Signal sequence‐independent targeting of MID 2 mRNA to the endoplasmic reticulum by the yeast RNA ‐binding protein Khd1p
Author(s) -
Syed Muhammad Ibrahim,
Moorthy Balaji T.,
Jenner Andreas,
Fetka Ingrid,
Jansen RalfPeter
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13098
Subject(s) - endoplasmic reticulum , messenger rna , signal peptide , rna binding protein , microbiology and biotechnology , biology , rna , yeast , saccharomyces cerevisiae , biochemistry , peptide sequence , gene
Localization of mRNA s depends on specific RNA‐binding proteins ( RBP s) and critically contributes not only to cell polarization but also to basal cell function. The yeast RBP Khd1p binds to several hundred mRNA s, the majority of which encodes secreted or membrane proteins. We demonstrate that a subfraction of Khd1p associates with artificial liposomes and endoplasmic reticulum ( ER ), and that Khd1p endomembrane association is partially dependent on its binding to RNA . ER targeting of at least two mRNA s, MID 2 and SLG 1 / WSC 1 , requires KHD 1 but is independent of their translation. Together, our results suggest interdependence of Khd1p and mRNA for their targeting to the ER and presents additional evidence for signal sequence‐independent, RBP ‐mediated mRNA targeting.