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SPT 6 interacts with NSD 2 and facilitates interferon‐induced transcription
Author(s) -
Ouda Ryota,
Sarai Naoyuki,
Nehru Vishal,
Patel Mira C.,
Debrosse Maxime,
Bachu Mahesh,
Chereji Răzvan V.,
Eriksson Peter R.,
Clark David J.,
Ozato Keiko
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13069
Subject(s) - transcription (linguistics) , housekeeping gene , interferon , gene , histone , chaperone (clinical) , microbiology and biotechnology , chemistry , small interfering rna , biology , rna , gene expression , genetics , philosophy , medicine , linguistics , pathology
The role of the histone chaperone SPT 6 in mammalian cells is not fully understood. Here, we investigated the involvement of SPT 6 in type I interferon ( IFN )‐induced transcription in murine fibroblasts. In RNA ‐seq analysis, Spt6 si RNA attenuates about half of ~ 200 IFN ‐stimulated genes ( ISG s), while not affecting housekeeping genes. ISG s with high mRNA induction are more susceptible to Spt6 si RNA than those with lower levels of induction. Ch IP analysis shows that SPT 6 is recruited to highly inducible, Spt6 si RNA ‐sensitive ISG s, but not to other si RNA ‐insensitive ISG s. Furthermore, SPT 6 recruitment is abrogated in cells lacking the histone methyltransferase NSD 2. In co‐ IP experiments, SPT 6 interacts with NSD 2. In summary, SPT 6 facilitates IFN ‐induced transcription, highlighting its critical role in gene activation.