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Engineering isoprene synthesis in cyanobacteria
Author(s) -
Chaves Julie E.,
Melis Anastasios
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13052
Subject(s) - isoprene , photosynthesis , renewable energy , terpenoid , synthetic biology , metabolic engineering , cyanobacteria , raw material , biochemical engineering , photobioreactor , chemistry , environmental science , biomass (ecology) , biology , ecology , engineering , organic chemistry , computational biology , biochemistry , bacteria , genetics , copolymer , enzyme , polymer
The renewable production of isoprene (Isp) hydrocarbons, to serve as fuel and synthetic chemistry feedstock, has attracted interest in the field recently. Isp (C 5 H 8 ) is naturally produced from sunlight, CO 2 and H 2 O photosynthetically in terrestrial plant chloroplasts via the terpenoid biosynthetic pathway and emitted in the atmosphere as a response to heat stress. Efforts to institute a high capacity continuous and renewable process have included heterologous expression of the Isp synthesis pathway in photosynthetic microorganisms. This review examines the premise and promise emanating from this relatively new research effort. Also examined are the metabolic engineering approaches applied in the quest of renewable Isp hydrocarbons production, the progress achieved so far, and barriers encountered along the way.