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Codon harmonization – going beyond the speed limit for protein expression
Author(s) -
Mig Charlotte,
Mariano Natacha,
Stadthagen Gustavo,
Lugari Adrien,
Lagoutte Priscillia,
Donnat Stéphanie,
Chenavas Sylvie,
Perot Cyril,
Sodoyer Régis,
Werle Bettina
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13046
Subject(s) - codon usage bias , computational biology , biology , gene , genetics , genome
Codon usage distribution has been soundly used by nature to fine tune protein biogenesis. Alteration of the mRNA structure or sequential scheduling of codons can profoundly affect translation, thus altering protein yield, functionality, solubility, and proper folding. Building on these observations, here, we present an evaluation of different recently designed algorithms of sequence adaptation based on Codon Adaptation Index ( CAI ) profiling. The first algorithm globally harmonizes synonymous codons in the original sequence in full respect to the heterologous expression host codon usage. The second recodes the sequence in accordance with the native sequence CAI profile. Our data, generated on three model proteins, highlights the importance to consider gene recoding as a parameter itself for recombinant protein expression improvement.