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Pseudomonas pseudoalcaligenes KF 707 grown with biphenyl expresses a cytochrome caa 3 oxidase that uses cytochrome c 4 as electron donor
Author(s) -
Sandri Federica,
Musiani Francesco,
Selamoglu Nur,
Daldal Fevzi,
Zani Davide
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13001
Subject(s) - thermus thermophilus , cytochrome c oxidase , cytochrome , chemistry , cytochrome c , biochemistry , peroxidase , oxidase test , cytochrome c peroxidase , biphenyl , microbiology and biotechnology , biology , enzyme , organic chemistry , mitochondrion , escherichia coli , gene
Combining peroxidase activity‐based heme staining ( TMBZ / SDS / PAGE ) with mass spectrometry analyses (Nano LC ‐ MS / MS ) of protein extracts from wild‐type and appropriate mutants, we provide evidence that the polychlorinated biphenyl degrader Pseudomonas pseudoalcaligenes KF 707 primarily expresses a caa 3 ‐type cytochrome c oxidase ( caa 3 ‐Cox) using cytochrome (cyt) c 4 as an electron donor in cells grown with biphenyl versus glucose as the sole carbon source. Homology modeling of KF 707 caa 3 ‐Cox using the three‐dimensional structure of that from Thermus thermophilus highlights multiple similarities and differences between the proton channels in subunit I of the aa 3 ‐ and caa 3 ‐Cox of Paracoccus and Thermus spp., respectively. To our knowledge, this is the first report demonstrating the presence of a caa 3 ‐Cox using cyt c 4 as an electron donor in a Pseudomonas species.