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Regulation of glucose‐stimulated insulin secretion by ATP ase Inhibitory Factor 1 ( IF 1)
Author(s) -
Kahancová Anežka,
Sklenář Filip,
Ježek Petr,
Dlasková Andrea
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12991
Subject(s) - secretion , endogeny , atp synthase , regulator , insulin , endocrinology , biology , chemistry , medicine , carbohydrate metabolism , biochemistry , enzyme , gene
ATP ase Inhibitory factor 1 ( IF 1) is an endogenous regulator of mitochondrial ATP synthase, which is involved in cellular metabolism. Although great progress has been made, biological roles of IF 1 and molecular mechanisms of its action are still to be elucidated. Here, we show that IF 1 is present in pancreatic β‐cells, bound to the ATP synthase also under normal physiological conditions. IF 1 silencing in model pancreatic β‐cells ( INS ‐1E) increases insulin secretion over a range of glucose concentrations. The left‐shifted dose–response curve reveals excessive insulin secretion even under low glucose, corresponding to fasting conditions. A parallel increase in cellular respiration and ATP levels is observed. To conclude, our results indicate that IF 1 is a negative regulator of insulin secretion involved in pancreatic β‐cell glucose sensing.