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A new class of genic nuclear RNA species in Arabidopsis
Author(s) -
Wonterghem Miranda,
Thieffry Axel,
Boyd Mette,
Bornholdt Jette,
Brodersen Peter
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12981
Subject(s) - rna , polyadenylation , biology , biogenesis , gene , genetics , methylation , locus (genetics) , small nuclear rna , microbiology and biotechnology , biochemistry , non coding rna
Targeting of Arabidopsis PHABULOSA ( PHB ) mRNA by miR166 has been implicated in gene body methylation at the PHB locus. We report that the PHB locus produces an array of stable nuclear RNA species that are neither polyadenylated nor capped. Their biogenesis requires neither RNA polymerases IV /V nor miR166‐guided cleavage. The PHB RNA s are insensitive to mutation of nuclear RNA decay pathways and are conserved in several Brassicaceae species, suggesting functional relevance. Similar RNA species are also produced by another body‐methylated locus encoding the miR414 target eIF 2. Our data reveal the existence of a new class of genic nuclear RNA species.