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Interplay of SpkG kinase and the Slr0151 protein in the phosphorylation of ferredoxin 5 in Synechocystis sp. strain PCC 6803
Author(s) -
Angeleri Martina,
Zorina Anna,
Aro EvaMari,
Battchikova Natalia
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12970
Subject(s) - phosphorylation , phosphoprotein , ferredoxin , synechocystis , protein phosphorylation , gene , kinase , biochemistry , gene cluster , biology , protein kinase a , chemistry , microbiology and biotechnology , enzyme , mutant
In Synechocystis 6803, the ferredoxin 5 (Fd5) phosphoprotein and the S/T protein kinase SpkG are encoded by the slr0148 and slr0152 genes, respectively, which belong to the slr0144–slr0152 cluster. Using a targeted proteomic approach, we showed that SpkG is responsible for the phosphorylation of Fd5 on residues T18 and T72. Sequence alignments and Fd5 structure modelling suggest that these phosphorylation events modulate protein–protein interaction. Furthermore, Fd5 phosphorylation is affected by the Slr0151 protein encoded by the gene preceding spkG in the gene cluster. We propose that Slr0151 functions as an auxiliary protein in the regulation of the ratio between phosphorylated and nonphosphorylated forms of Fd5.