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Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth
Author(s) -
Sede Ana R.,
Borassi Cecilia,
Wengier Diego L.,
Mecchia Martín A.,
Estevez José M.,
Muschietti Jorge P.
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12947
Subject(s) - pollen tube , callose , pollen , arabidopsis , arabidopsis thaliana , leucine rich repeat , tip growth , mutant , biology , microbiology and biotechnology , cell wall , germination , botany , biochemistry , gene , kinase , pollination
Proper cell wall assembly is crucial during pollen tube growth. Leucine‐rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline‐rich glycoprotein (HRGP) family. They contain a conserved N‐terminal leucine‐rich repeat (LRR) domain and a highly variable C‐terminal extensin domain. Here, we characterized four LRX proteins (LRX8 through LRX11) from pollen of Arabidopsis thaliana . To investigate the role of LRX8‐LRX11 in pollen germination and pollen tube growth, multiple T‐DNA lrx mutants were obtained. The lrx mutants display abnormal pollen tubes with an irregular deposition of callose and pectin. They also show serious alterations in pollen germination and segregation ratio. Our results suggest that LRXs are involved in ensuring proper cell wall assembly during pollen tube growth.