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The expression of the embryonic gene Cripto‐1 is regulated by OCT 4 in human embryonal carcinoma NCCIT cells
Author(s) -
Park SungWon,
Do HyunJin,
Han MiHee,
Choi Wonbin,
Kim JaeHwan
Publication year - 2018
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12935
Subject(s) - gene knockdown , embryonic stem cell , biology , transactivation , activator (genetics) , microbiology and biotechnology , small hairpin rna , promoter , gene expression , transcription factor , gene , cancer research , genetics
Cripto‐1 and OCT 4, expressed in stem cells and cancers, play important roles in tumorigenesis. Here, we demonstrate that Cripto‐1 expression is regulated by OCT 4 in human embryonic carcinoma NCCIT cells. The endogenous expression of Cripto‐1 and OCT 4 is significantly reduced during differentiation. Cripto‐1 expression is increased by OCT 4 overexpression, but decreased by sh RNA ‐mediated OCT 4 knockdown. OCT 4 overexpression significantly activates Cripto‐1 transcriptional activity. A 5′‐upstream minimal promoter sequence in the gene‐encoding Cripto‐1 is significantly activated by OCT 4 overexpression. Mutation of the putative OCT 4‐binding site abolishes OCT 4‐mediated activation of the Cripto‐1 promoter. The OCT 4 transactivation domains mediate transcriptional activity of the Cripto‐1 minimal promoter through direct interaction. Taken together, OCT 4 plays an important role as a transcriptional activator of Cripto‐1 expression in NCCIT cells.