Premium
Nop5 interacts with the archaeal RNA exosome
Author(s) -
Gauernack A. Susann,
Lassek Christian,
Hou Linlin,
Dzieciolowski Julia,
EvguenievaHackenberg Elena,
Klug Gabriele
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12915
Subject(s) - exosome complex , rna , exosome , ribonucleoprotein , sulfolobus solfataricus , microbiology and biotechnology , polyadenylation , rna binding protein , biology , chemistry , biochemistry , non coding rna , microvesicles , gene , microrna , archaea
The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA , has an RNA ‐binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the exosome of Sulfolobus solfataricus grown to a late stationary phase. We demonstrated the capability of Nop5 to bind to the exosome with a homotrimeric Rrp4‐cap and to increase the proportion of polyadenylated RNA in vitro , suggesting that Nop5 is a dual‐function protein. Since tailing of RNA probably serves to enhance RNA degradation, association of Nop5 with the archaeal exosome in the stationary phase may enhance tailing and degradation of RNA as survival strategy.