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Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms
Author(s) -
Shwab Elliot K.,
Juvvadi Praveen R.,
Waitt Greg,
Soderblom Erik J.,
Moseley Martin A.,
Nicely Nathan I.,
Steinbach William J.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12886
Subject(s) - aspergillus fumigatus , phosphorylation , protein subunit , biology , protein phosphorylation , fungal protein , virulence , protein kinase a , microbiology and biotechnology , cell wall , biochemistry , saccharomyces cerevisiae , yeast , gene
Protein kinase A ( PKA ) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus . Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography‐tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subunit PkaR, distinct from those previously identified in mammals and yeasts, and demonstrated the importance of two phosphorylation clusters for hyphal growth and cell wall‐stress response. We also identified key differences in the regulation of PKA subcellular localization in A. fumigatus compared with other species. This is the first analysis of the phosphoregulation of a PKA regulatory subunit in a filamentous fungus and uncovers critical mechanistic differences between PKA regulation in filamentous fungi compared with mammals and yeast species, suggesting divergent targeting opportunities.