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Characterization of conserved circular RNA in polyploid Gossypium species and their ancestors
Author(s) -
Zhao Ting,
Wang Luyao,
Li Sai,
Xu Min,
Guan Xueying,
Zhou Baoliang
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12868
Subject(s) - rna , gossypium , biology , intron , polyploid , genetics , exon , non coding rna , ploidy , circular rna , rna splicing , microbiology and biotechnology , gene
Circular RNA (circ RNA ) is a regulatory class of long, noncoding RNA found in both plant and animal kingdoms. The profile and characterization of circ RNA in cotton species remains to be explored. Here, using 24 rRNA ‐depleted RNA ‐seq libraries of putative diploid progenitors of Gossypium spp., Gossypium arboreum and Gossypium raimondii , their interspecies hybrid (F 1 ) and allotetraploid Gossypium hirsutum , 1041, 1478, 1311, and 499 circ RNA s were identified in each cotton species, respectively. A prevalence of 23 exon‐circ RNA s contain noncanonical GT / AG signals, and only ~ 10% of exon‐circ RNA is associated with reverse complementary intronic sequences. This result implies that plants employ a method of circ RNA splicing distinct from that of animals. In addition, 432 circ RNA s are stably expressed in multiple cotton species.