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Measurement of 13 C turnover into glutamate and glutamine pools in brain tumor patients
Author(s) -
Pichumani Kumar,
Mashimo Tomoyuki,
Vemireddy Vamsidhara,
Ijare Omkar B.,
Mickey Bruce E.,
Malloy Craig R.,
MarinValencia Isaac,
Baskin David S.,
Bachoo Robert M.,
Maher Elizabeth A.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12867
Subject(s) - glutamine , glutamate receptor , chemistry , biochemistry , radiochemistry , amino acid , receptor
Malignant brain tumors are known to utilize acetate as an alternate carbon source in the citric acid cycle for their bioenergetics. 13 C NMR ‐based isotopomer analysis has been used to measure turnover of 13 C‐acetate carbons into glutamate and glutamine pools in tumors. Plasma from the patients infused with [1,2‐ 13 C]acetate further revealed the presence of 13 C isotopomers of glutamine, glucose, and lactate in the circulation that were generated due to metabolism of [1,2‐ 13 C]acetate by peripheral organs. In the tumor cells, [4‐ 13 C] and [3,4‐ 13 C]glutamate and glutamine isotopomers were generated from blood‐borne 13 C‐labeled glucose and lactate which were formed due to [1,2‐ 13 C[acetate metabolism of peripheral tissues. [4,5‐ 13 C] and [3,4,5‐ 13 C]glutamate and glutamine isotopomers were produced from [1,2‐ 13 C]acetyl‐CoA that was derived from direct oxidation of [1,2‐ 13 C] acetate in the tumor. Major portion of C4 13 C fractional enrichment of glutamate (93.3 ± 0.02%) and glutamine (90.9 ± 0.03%) were derived from [1,2‐ 13 C]acetate‐derived acetyl‐CoA.