Premium
An in vivo proteomic analysis of the Me31B interactome in Drosophila germ granules
Author(s) -
DeHaan Hunter,
McCambridge Aidan,
Armstrong Brittany,
Cruse Carlie,
Solanki Dhruv,
Trinidad Jonathan C.,
Arkov Alexey L.,
Gao Ming
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12854
Subject(s) - interactome , germ plasm , biology , germ cell , microbiology and biotechnology , ribonucleoprotein , rna binding protein , proteomics , rna , genetics , gene , embryo
Drosophila Me31B is a conserved protein of germ granules, ribonucleoprotein complexes essential for germ cell development. Me31B post‐transcriptionally regulates mRNAs by interacting with other germ granule proteins. However, a Me31B interactome is lacking. Here, we use an in vivo proteomics approach to show that the Me31B interactome contains polypeptides from four functional groups: RNA regulatory proteins, glycolytic enzymes, cytoskeleton/motor proteins, and germ plasm components. We further show that Me31B likely colocalizes with the germ plasm components Tudor (Tud), Vasa, and Aubergine in the nuage and germ plasm and provide evidence that Me31B may directly bind to Tud in a symmetrically dimethylated arginine‐dependent manner. Our study supports the role of Me31B in RNA regulation and suggests its novel roles in germ granule assembly and function.