Premium
The α1β1 region is crucial for biofilm enhancement activity of MTC 28 in Mycobacterium smegmatis
Author(s) -
Kundu Prasun,
Dutta Debabrata,
Kumar Das Amit
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12823
Subject(s) - mycobacterium smegmatis , biofilm , mycobacterium tuberculosis , flow cytometry , microbiology and biotechnology , chemistry , cell wall , mycobacterium , bacteria , biology , tuberculosis , biochemistry , genetics , medicine , pathology
We show here that MTC28, a secretory antigen of 28 kD a from Mycobacterium tuberculosis , is involved in biofilm formation. The exogenous addition of MTC 28 to the culture medium as well its expression in Mycobacterium smegmatis mc 2 155 shows an enhancement in biofilm formation, which leads to drug resistance. Structural analysis of MTC 28 followed by mutational studies confirms the role of its α1β1 region in the biofilm enhancement activity. Confocal and flow cytometry studies show that the α1β1 region of MTC 28 is crucial for binding to the M. smegmatis cell wall. The enhancement in biofilm formation due to MTC 28 is also observed in M. tuberculosis H37Ra. This is the first report on the structure–function relationship of MTC 28.