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Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells
Author(s) -
Saito Yuki,
Kunitomi Akira,
Seki Tomohisa,
Tohyama Shugo,
Kusumoto Dai,
Takei Makoto,
Kashimura Shin,
Hashimoto Hisayuki,
Yozu Gakuto,
Motoda Chikaaki,
Shimojima Masaya,
Egashira Toru,
Oda Mayumi,
Fukuda Keiichi,
Yuasa Shinsuke
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12791
Subject(s) - homeobox protein nanog , epigenetics , rex1 , embryonic stem cell , biology , gene expression , dnmt1 , stem cell , microbiology and biotechnology , regulation of gene expression , dna methylation , gene , nanog homeobox protein , induced pluripotent stem cell , genetics
The expression of pluripotency genes fluctuates in a population of embryonic stem (ES) cells and the fluctuations in the expression of some pluripotency genes correlate. However, no correlation in the fluctuation of Pou5f1 , Zfp42 , and Nanog expression was observed in ES cells. Correlation between Pou5f1 and Zfp42 fluctuations was demonstrated in ES cells containing a knockout in the NuRD component Mbd3 . ES cells containing a triple knockout in the DNA methyltransferases Dnmt1 , Dnmt3a , and Dnmt3b showed correlation between the fluctuation of Pou5f1 , Zfp42 , and Nanog gene expression. We suggest that an epigenetic barrier is key to preventing the propagation of fluctuating pluripotency gene expression in ES cells.