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Regulation of Rab5 isoforms by transcriptional and post‐transcriptional mechanisms in yeast
Author(s) -
Schmidt Oliver,
Weyer Yannick,
Fink Matthias J.,
Müller Martin,
Weys Sabine,
Bindreither Marietta,
Teis David
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12785
Subject(s) - biogenesis , microbiology and biotechnology , saccharomyces cerevisiae , gene isoform , biology , untranslated region , messenger rna , transcription (linguistics) , endosome , transcriptional regulation , yeast , transcription factor , gene , genetics , linguistics , philosophy , intracellular
Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins: Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels increase but never exceed that of Vps21. Its induction requires the transcription factors Crz1 and Gis1. In growing cells, the expression of Ypt53 is suppressed by post‐transcriptional mechanisms mediated by the untranslated regions of the YPT53 mRNA. Based on genetic experiments, these sequences appear to stimulate deadenylation, Pat1‐activated decapping and Xrn1‐mediated mRNA degradation. Once this regulation is bypassed, Ypt53 protein levels surpass Vps21, and Ypt53 is sufficient to maintain endosomal function and cell growth.