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Dissecting the variation in transcriptional circuits between naive and primed pluripotent states
Author(s) -
Deb Aritra,
Sarkar Arijita,
Ghosh Zhumur
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12732
Subject(s) - induced pluripotent stem cell , variation (astronomy) , biology , genetics , microbiology and biotechnology , computational biology , gene , physics , embryonic stem cell , astrophysics
Naive and primed pluripotent states are very similar to each other, but subtle differences exist in their maintenance and differentiation programmes. Transcription factors ( TF s) play a key role towards maintaining pluripotency and cellular reprogramming. However, TF expression dynamics and regulatory mechanisms in naive and primed pluripotent states are poorly understood. Here, we performed a comprehensive transcriptional analysis of both states, which revealed a gene expression pattern in mESC s (naive state) that appear to be distinct from mE pi SC s (primed state). We screened 10 TF s essential for maintenance, self‐renewal and differentiation, of which the TF s‐ Notch3, Meis1, Gli3 and Srf can act as novel markers distinguishing the two states. Furthermore, a detailed bioinformatic analysis (involving these TF s) elucidated essential transcriptional circuits between the naive and primed pluripotent states.