Premium
Subconductance states of mitochondrial chloride channels: implication for functionally‐coupled tetramers
Author(s) -
Tomasek Milan,
Misak Anton,
Grman Marian,
Tomaskova Zuzana
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12721
Subject(s) - gating , mitochondrion , chloride channel , biophysics , lipid bilayer , conductance , intracellular , chemistry , inner mitochondrial membrane , chloride , membrane , biochemistry , biology , physics , organic chemistry , condensed matter physics
Recently, it has been discovered that isoforms of intracellular chloride channels (CLIC) are present in cardiac mitochondria. By reconstituting rat cardiac mitochondrial chloride channels into bilayer lipid membranes, we detected three equally separated subconductance states with conductance increment of 45 pS and < 2% occupancy. The observed rare events of channel decomposition into substates, accompanied by disrupted gating, provide an insight into channel quaternary structure. Our findings suggest that the observed channels work as four functionally coupled subunits with synchronized gating. We discuss the putative connection of channel activity from native mitochondria with the recombinant CLIC channels. However, conclusive evidence is needed to prove this connection.