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Single‐cell analyses to reveal hematopoietic stem cell fate decisions
Author(s) -
Lunger Ilaria,
Fawaz Malak,
Rieger Michael A.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12712
Subject(s) - stem cell , haematopoiesis , hematopoietic stem cell , hematopoietic cell , cell , microbiology and biotechnology , cell fate determination , chemistry , biology , biochemistry , transcription factor , gene
Hematopoietic stem cells ( HSC s) are the best studied adult stem cells with enormous clinical value. Most of our knowledge about their biology relies on assays at the single HSC level. However, only the recent advances in developing new single cell technologies allowed the elucidation of the complex regulation of HSC fate decision control. This Review will focus on current attempts to investigate individual HSC s at molecular and functional levels. The advantages of these technologies leading to groundbreaking insights into hematopoiesis will be highlighted, and the challenges facing these technologies will be discussed. The importance of combining molecular and functional assays to enlighten regulatory networks of HSC fate decision control, ideally at high temporal resolution, becomes apparent for future studies.