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The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK 33
Author(s) -
CorratgéFaillie Claire,
Ronzier Elsa,
Sanchez Frédéric,
Prado Karine,
Kim JeongHyeon,
Lanciano Sophie,
Leonhardt Nathalie,
Lacombe Benoît,
Xiong Tou Cheu
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12687
Subject(s) - guard cell , shaker , microbiology and biotechnology , potassium channel , phosphatase , kinase , arabidopsis , xenopus , phosphorylation , chemistry , biology , mutant , biochemistry , biophysics , gene , physics , quantum mechanics , vibration
A complex signaling network involving voltage‐gated potassium channels from the Shaker family contributes to the regulation of stomatal aperture. Several kinases and phosphatases have been shown to be crucial for ABA ‐dependent regulation of the ion transporters. To date, the Ca 2+ ‐dependent regulation of Shaker channels by Ca 2+ ‐dependent protein kinases ( CPK s) is still elusive. A functional screen in Xenopus oocytes was launched to identify such CPK s able to regulate the three main guard cell Shaker channels KAT 1, KAT 2, and GORK . Seven guard cell CPK s were tested and multiple CPK /Shaker couples were identified. Further work on CPK 33 indicates that GORK activity is enhanced by CPK 33 and unaffected by a nonfunctional CPK 33 ( CPK 33‐K102M). Furthermore, Ca 2+ ‐induced stomatal closure is impaired in two cpk33 mutant plants.