Premium
Tonoplast ( Bv TIP 1;2) and plasma membrane ( Bv PIP 2;1) aquaporins show different mechanosensitive properties
Author(s) -
Goldman Ramiro P.,
Jozefkowicz Cintia,
Canessa Fortuna Agustina,
Sutka Moira,
Alleva Karina,
Ozu Marcelo
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12671
Subject(s) - mechanosensitive channels , aquaporin , chemistry , membrane , biophysics , vacuole , microbiology and biotechnology , biochemistry , biology , ion channel , cytoplasm , receptor
Previous works proposed that aquaporins behave as mechanosensitive channels. However, principal issues about mechanosensitivity of aquaporins are not known. In this work, we characterized the mechanosensitive properties of the water channels Bv TIP 1;2 ( TIP 1) and Bv PIP 2;1 ( PIP 2) from red beet ( Beta vulgaris ). We simultaneously measured the mechanical behavior and the water transport rates during the osmotic response of emptied‐out oocytes expressing TIP 1 or PIP 2. Our results indicate that TIP 1 is a mechanosensitive aquaporin, whereas PIP 2 is not. We found that a single exponential function between the osmotic permeability coefficient and the volumetric elastic modulus governs the mechanosensitivity of TIP 1. Finally, homology modeling analysis indicates that putative residues involved in mechanosensitivity show different quantity and distribution in TIP 1 and PIP 2.