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Protein NCRII ‐18: the role of gene fusion in the molecular evolution of restriction endonucleases
Author(s) -
Ibryashkina Elena M.,
Solonin Alexander S.,
Zakharova Marina V.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12669
Subject(s) - chemistry , restriction enzyme , fusion protein , gene , biochemistry , biology , recombinant dna
This work first constructed the fusion protein NCRII ‐18 by fusing the restriction endonuclease Ecl18kI gene and part of the gene coding for the N‐terminal domain of the endonuclease EcoRII. The fusion of the EcoRII N‐terminal domain leads to a change in the properties of the recombinant protein. Unlike Ecl18kI, which made the basis of NCRII ‐18, the fusion protein predominantly recognizes the CCWGG sites, having lost the capability of interacting with the CCSGG sites. Experimental data support the hypothesis of a close evolutionary relationship between type IIE and IIP restriction endonucleases via a recombination between domains with active site structure and elements for recognition with domains responsible for recognition of DNA sequences.