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The pathway intermediate 2‐keto‐3‐deoxy‐L‐galactonate mediates the induction of genes involved in D‐galacturonic acid utilization in Aspergillus niger
Author(s) -
Alazi Ebru,
Khosravi Claire,
Homan Tim G.,
Pré Saskia,
Arentshorst Mark,
Di Falco Marcos,
Pham Thi T. M.,
Peng Mao,
AguilarPontes Maria Victoria,
Visser Jaap,
Tsang Adrian,
Vries Ronald P.,
Ram Arthur F. J.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12654
Subject(s) - aspergillus niger , catabolism , mutant , biochemistry , sugar acids , gene , inducer , enzyme , biology , glyceraldehyde , chemistry , dehydrogenase , sugar
In Aspergillus niger , the enzymes encoded by gaaA , gaaB , and gaaC catabolize d ‐galacturonic acid ( GA ) consecutively into l‐ galactonate, 2‐keto‐3‐deoxy‐ l‐ galactonate, pyruvate, and l‐ glyceraldehyde, while GaaD converts l‐ glyceraldehyde to glycerol. Deletion of gaaB or gaaC results in severely impaired growth on GA and accumulation of l‐ galactonate and 2‐keto‐3‐deoxy‐ l‐ galactonate, respectively. Expression levels of GA ‐responsive genes are specifically elevated in the ∆gaaC mutant on GA as compared to the reference strain and other GA catabolic pathway deletion mutants. This indicates that 2‐keto‐3‐deoxy‐ l‐ galactonate is the inducer of genes required for GA utilization.