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Structural communication between the chromophore‐binding pocket and the N‐terminal extension in plant phytochrome phyB
Author(s) -
Velázquez Escobar Francisco,
Buhrke David,
Fernandez Lopez Maria,
Shenkutie Sintayehu Manaye,
Horsten Silke,
Essen LarsOliver,
Hughes Jon,
Hildebrandt Peter
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12642
Subject(s) - chromophore , phytochrome , chemistry , isomerization , resonance raman spectroscopy , stereochemistry , hydrogen bond , kinetics , biophysics , photochemistry , raman spectroscopy , molecule , biochemistry , catalysis , biology , red light , botany , physics , organic chemistry , quantum mechanics , optics
The N‐terminal extension ( NTE ) of plant phytochromes has been suggested to play a functional role in signaling photoinduced structural changes. Here, we use resonance Raman spectroscopy to study the effect of the NTE on the chromophore structure of B‐type phytochromes from two evolutionarily distant plants. NTE deletion seems to have no effect on the chromophore in the inactive Pr state, but alters the torsion of the C‐D ring methine bridge and the surrounding hydrogen bonding network in the physiologically active Pfr state. These changes are accompanied by a shift of the conformational equilibrium between two Pfr substates, which might affect the thermal isomerization rate of the C‐D double bond and, thus, account for the effect of the NTE on the dark reversion kinetics.