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The alternative life of RNA —sequencing meets single molecule approaches
Author(s) -
FernándezMoya Sandra M.,
Ehses Janina,
Kiebler Michael A.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12639
Subject(s) - polyadenylation , gene isoform , rna splicing , rna , computational biology , alternative splicing , ribonucleoprotein , biology , untranslated region , messenger rna , rna binding protein , microbiology and biotechnology , function (biology) , gene , genetics
The central dogma of RNA processing has started to totter. Single genes produce a variety of mRNA isoforms by mRNA modification, alternative polyadenylation (APA), and splicing. Different isoforms, even those that code for the identical protein, may differ in function or spatiotemporal expression. One option of how this can be achieved is by the selective recruitment of trans ‐acting factors to the 3′‐untranslated region of a given isoform. Recent innovations in high‐throughput RNA ‐sequencing methods allow deep insight into global RNA regulation, whereas novel imaging‐based technologies enable researchers to explore single RNA molecules during different stages of development, in different tissues and different compartments of the cell. Resolving the dynamic function of ribonucleoprotein particles in splicing, APA, or RNA modification will enable us to understand their contribution to pathological conditions.