Premium
Aggregation of Mod5 is affected by tRNA binding with implications for tRNA gene‐mediated silencing
Author(s) -
Read David F.,
Waller Thomas J.,
Tse Eric,
Southworth Daniel R.,
Engelke David R.,
Smaldino Philip J.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12627
Subject(s) - transfer rna , gene silencing , gene , rna , biology , function (biology) , microbiology and biotechnology , genetics
Mod5 is a multifunctional protein that modifies a subset of tRNA s in the cytoplasm and is also required for an RNA ‐mediated form of transcriptional silencing. Previous in vivo studies have shown that the nuclear silencing function of Mod5 does not require that the causative tRNA gene encode a Mod5 substrate, although Mod5 is still required. However, previous data have not directly tested whether Mod5 can directly bind substrate and nonsubstrate RNA s. We herein demonstrate that Mod5 directly binds to both substrate and nonsubstrate RNA s, including a highly structured, non‐ tRNA sequence (5S‐ rRNA ), consistent with previous in vivo data. Furthermore, we show that some RNA s drastically change the aggregation behavior of Mod5 with implications for tRNA gene‐mediated silencing.