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Gfh factors and NusA cooperate to stimulate transcriptional pausing and termination
Author(s) -
Agapov Aleksei,
Olina Anna,
Esyunina Daria,
Kulbachinskiy Andrey
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12609
Subject(s) - rna polymerase , rna , deinococcus radiodurans , microbiology and biotechnology , small hairpin rna , transcription factor , transcription (linguistics) , biology , chemistry , genetics , gene , linguistics , philosophy
Lineage‐specific Gfh factors from the radioresistant bacterium Deinococcus radiodurans , which bind within the secondary channel of RNA polymerase, stimulate transcriptional pausing at a wide range of pause signals (elemental, hairpin‐dependent, post‐translocated, backtracking‐dependent, and consensus pauses) and increase intrinsic termination. Universal bacterial factor NusA, which binds near the RNA exit channel, enhances the effects of Gfh factors on termination and hairpin‐dependent pausing but do not act on other pause sites. It is proposed that NusA and Gfh target different steps in the pausing pathway and may act together to regulate transcription under stress conditions. Thus, transcription factors that interact with nascent RNA in the RNA exit channel can communicate with secondary channel regulators to modulate RNA polymerase activities.