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Prdx1‐ encoded peroxiredoxin is important for vascular development in zebrafish
Author(s) -
Huang PoChun,
Chiu ChienChih,
Chang HsuehWei,
Wang YiShan,
Syue HaiHong,
Song YiChun,
Weng ZhiHong,
Tai MingHong,
Wu ChangYi
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12604
Subject(s) - gene knockdown , zebrafish , microbiology and biotechnology , oxidative stress , peroxiredoxin , homeostasis , biology , function (biology) , chemistry , biochemistry , gene , peroxidase , enzyme
Genetic signaling and redox homeostasis are required for proper growth of blood vessels. Here, we report a novel function of peroxiredoxin1 (Prdx1) in vascular development in zebrafish. Knockdown of prdx1 impairs the growth of intersegmental vessel and caudal vein plexus (CVP), and reduces the expression of vascular markers, thus suggesting a role for prdx1 in vasculature and indicating that the antioxidant function of prdx1 is important. We found that H 2 O 2 ‐treated embryos also have CVP defects and observed synergistic effects when prdx1 knockdown was combined with H 2 O 2 treatment. Moreover, N ‐acetyl‐cysteine treatment rescues the vascular defects in prdx1 morphants. These results suggest that oxidative stress disturbs vascularization. Furthermore, we show that the regulation of prdx1 is mediated by Notch and BMP signals.