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Characterizing isomiR variants within the micro RNA ‐34/449 family
Author(s) -
Mercey Olivier,
Popa Alexandra,
Cavard Amélie,
Paquet Agnès,
Chevalier Benoît,
Pons Nicolas,
Mag Virginie,
Zangari Joséphine,
Brest Patrick,
Zaragosi LaureEmmanuelle,
Ponzio Gilles,
Lebrigand Kevin,
Barbry Pascal,
Marcet Brice
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12595
Subject(s) - non canonical , rna , biology , gene , microbiology and biotechnology , genetics , gene expression
miR‐34/449 micro RNA s are conserved regulators of multiciliated cell differentiation. Here, we evidence and characterize expression of two isomiR variant sequences from the miR‐34/449 family in human airway epithelial cells. These isomiRs differ from their canonical counterparts miR‐34b and miR‐449c by one supplemental uridine at their 5′‐end, leading to a one‐base shift in their seed region. Overexpression of canonical miR‐34/449 or 5′‐isomiR‐34/449 induces distinct gene expression profiles and biological effects. However, some target transcripts and functional activities are shared by both canonical micro RNA s and isomiRs. Indeed, both repress important targets that result in cell cycle blockage and Notch pathway inhibition. Our findings suggest that 5′‐isomiR‐34/449 may represent additional mechanisms by which miR‐34/449 family finely controls several pathways to drive multiciliogenesis.