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The deubiquitinating enzyme Usp5 regulates Notch and RTK signaling during Drosophila eye development
Author(s) -
Ling Xuemei,
Huang Qinzhu,
Xu Yanqin,
Jin Yuxiao,
Feng Ying,
Shi Weijie,
Ye Xiaolei,
Lin Yi,
Hou Ling,
Lin Xinhua
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12580
Subject(s) - deubiquitinating enzyme , hairless , notch signaling pathway , downregulation and upregulation , microbiology and biotechnology , ubiquitin , gene knockdown , biology , signal transduction , genetics , gene
Usp5 belongs to the USP family of deubiquitinating enzymes ( DUB s), which comprises the largest class of DUB s. We previously reported that loss of Usp5 impairs development of photoreceptors in Drosophila eyes, although the detailed mechanism remained unclear. In the present study, we demonstrate that Usp5 regulates both Notch and receptor tyrosine kinase ( RTK ) signaling. Loss of Usp5 results in upregulation of Notch signaling and downregulation of RTK signaling, leading to impaired photoreceptor development. Moreover, genetic rescue experiments with the DNA binding protein Suppressor of Hairless or Notch RNA i indicate that they mediate the regulation of RTK signaling by Usp5. The present study provides mechanistic insight into how Usp5 regulates photoreceptor differentiation by Notch and RTK signaling in the Drosophila eye.