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Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest‐like precursors
Author(s) -
Takayama Yuzo,
Wakabayashi Tamami,
Kushige Hiroko,
Saito Yutaka,
Shibuya Yoichiro,
Shibata Shinsuke,
Akamatsu Wado,
Okano Hideyuki,
Kida Yasuyuki S.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12572
Subject(s) - neural crest , microbiology and biotechnology , embryonic stem cell , biology , enhancer , population , precursor cell , cell , gene expression , embryo , gene , biochemistry , demography , sociology
In this study, we propose a novel method for inducing neuronal cells by briefly exposing them to small‐molecule cocktails in a step‐by‐step manner. Global gene expression analysis with immunohistochemical staining and calcium flux assays reveal the generation of neurons from mouse embryonic fibroblasts. In addition, time‐lapse imaging of neural precursor‐specific enhancer expression and global gene expression analyses show that the neurons are generated by passing through a neural crest‐like precursor stage. Consistent with these results, the neural crest‐like cells are able to differentiate into neural crest lineage cells, such as sympathetic neurons, adipocytes, osteocytes, and smooth muscle cells. Therefore, these results indicate that brief exposure to chemical compounds could expand and induce a substantial multipotent cell population without viral transduction.