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Bidirectional communication between sensory neurons and osteoblasts in an in vitro coculture system
Author(s) -
Kodama Daisuke,
Hirai Takao,
Kondo Hisataka,
Hamamura Kazunori,
Togari Akifumi
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12561
Subject(s) - in vitro , microbiology and biotechnology , sensory system , chemistry , osteoblast , neuroscience , biology , biochemistry
Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion ( DRG ) and the osteoblast‐like MC 3T3‐E1 cells using an in vitro coculture system. Our findings indicate that signal transduction from DRG ‐derived neurons to MC 3T3‐E1 cells is suppressed by antagonists of the AMPA receptor and the NK 1 receptor. Conversely, signal transduction from MC 3T3‐E1 cells to DRG ‐derived neurons is suppressed by a P2X 7 receptor antagonist. Our results suggest that these cells communicate with each other by exocytosis of glutamate, substance P in the efferent signal, and ATP in the afferent signal.