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Crystal structure of a family 80 chitosanase from Mitsuaria chitosanitabida
Author(s) -
Yorinaga Yutaka,
Kumasaka Takashi,
Yamamoto Masaki,
Hamada Kensaku,
Kawamukai Makoto
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12557
Subject(s) - chitosanase , glycoside hydrolase , hydrolase , chemistry , glucosamine , chitin , biochemistry , enzyme , stereochemistry , acetylation , chitosan , gene
Chitosanases belong to glycoside hydrolase families 5, 7, 8, 46, 75 and 80 and hydrolyse glucosamine polymers produced by partial or full deacetylation of chitin. Herein, we determined the crystal structure of chitosanase from the β‐proteobacterium, Mitsuaria chitosanitabida , (McChoA) at 1.75 Å resolution; the first structure of a family 80 chitosanase. McChoA is a 34 kDa extracellular protein of 301 amino acids that fold into two (upper and lower) globular domains with an active site cleft between them. Key substrate‐binding features are conserved with family 24 lysozymes and family 46 chitosanases. The distance between catalytic residues E41 and E61 (10.8 Å) indicates an inverting type mechanism. Uniquely, three disulphide bridges and the C terminus might contribute to enzyme activity.