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The amino acid transporter, SLC 1A3, is plasma membrane‐localised in adipocytes and its activity is insensitive to insulin
Author(s) -
Krycer James R.,
Fazakerley Daniel J.,
Cater Rosemary J.,
C. Thomas Kristen,
Naghiloo Sheyda,
Burchfield James G.,
Humphrey Sean J.,
Vandenberg Robert J.,
Ryan Renae M.,
James David E.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12549
Subject(s) - insulin , phosphorylation , transporter , biochemistry , xenopus , amino acid , chemistry , solute carrier family , catabolism , biology , endocrinology , metabolism , gene
The hormone insulin coordinates the catabolism of nutrients by protein phosphorylation. Phosphoproteomic analysis identified insulin‐responsive phosphorylation of the Glu/Asp transporter SLC 1A3/ EAAT 1 in adipocytes. The role of SLC 1A3 in adipocytes is not well‐understood. We show that SLC 1A3 is localised to the plasma membrane and the major regulator of acidic amino acid uptake in adipocytes. However, its localisation and activity were unaffected by insulin or mutation of the insulin‐regulated phosphosite. The latter was also observed using a heterologous expression system in Xenopus laevis oocytes. Thus, SLC 1A3 maintains a constant import of acidic amino acids independently of nutritional status in adipocytes.