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FGF 11 induced by hypoxia interacts with HIF ‐1α and enhances its stability
Author(s) -
Lee Kyeong Won,
Yim HyungSoon,
Shin Jihye,
Lee Cheolju,
Lee JungHyun,
Jeong JaeYeon
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12547
Subject(s) - immunoprecipitation , fibroblast growth factor , hypoxia (environmental) , microbiology and biotechnology , chemistry , gene knockdown , messenger rna , biology , biochemistry , receptor , gene , oxygen , organic chemistry
Fibroblast growth factor 11 ( FGF 11) is an intracellular FGF . Although induction of FGF 11 by hypoxia has been observed in several cell types, the molecular function of FGF 11 is not clearly understood yet. Here, we investigated the role of FGF 11 under hypoxia. We identified hypoxia‐inducible factor‐1α ( HIF ‐1α) as an interacting protein of FGF 11 using immunoprecipitation and mass spectrometry. FGF 11 knockdown decreased HIF ‐1α protein, while FGF 11 overexpression increased it, without affecting HIF ‐1α mRNA . Protein stability test and ubiquitination assay showed that FGF 11 increased HIF ‐1α stability by acting upstream of proteasomal degradation. Altogether, these results suggest a cross‐regulation between HIF ‐1α and FGF 11, through which hypoxia‐induced FGF 11 reinforces hypoxia responses by enhancing the stability of HIF ‐1α.