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Regulation of BC 200 RNA ‐mediated translation inhibition by hn RNP E1 and E2
Author(s) -
Jang Seonghui,
Shin Heegwon,
Lee Jungmin,
Kim Youngmi,
Bak Geunu,
Lee Younghoon
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12544
Subject(s) - rna , translation (biology) , rna binding protein , non coding rna , microbiology and biotechnology , biology , messenger rna , chemistry , biochemistry , gene
The long noncoding RNA BC 200 (brain cytoplasmic RNA , 200 nucleotides) acts as a translational modulator of local protein synthesis at dendrites. BC200 RNA has been shown to inhibit translation in vitro , but it remains unknown how this translation inhibition might be controlled in a cell. Here, we performed yeast three‐hybrid screening and identified hn RNP E1 and hn RNP E2 as BC200 RNA‐interacting proteins. We found that: these hn RNA proteins could restore BC200 RNA‐inhibited translation; BC200 RNA interacts with hn RNP E1 and E2 mainly through its unique 3′ C‐rich domain; and the RNA binding specificities and modes of the two proteins differed somewhat. Our results offer new insights into the regulation of BC200 RNA‐mediated translation inhibition.