Premium
A novel isoform of the human mitochondrial complex I subunit NDUFV 3
Author(s) -
Dibley Marris G.,
Ryan Michael T.,
Stroud David A.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12527
Subject(s) - gene isoform , protein subunit , chemistry , microbiology and biotechnology , biochemistry , biology , gene
Human mitochondrial complex I is the first enzyme of the mitochondrial respiratory chain. Complex I is composed of 45 subunits, seven encoded by mitochondrial DNA , while the remainder are encoded by nuclear DNA . All nuclear‐encoded subunits are thought to be expressed as a single isoform. Here we reveal subunit NDUFV 3 to be present in both the canonical 10 kD a and a novel 50 kD a isoform, generated through alternative splicing. Both isoforms assemble into complex I and their levels vary in different tissues. While the 50 kD a isoform appears to be dominant in HEK 293T cells, we find either isoform alone is sufficient for assembly of mature complex I. NDUFV 3 represents the first known complex I subunit present in two functional isoforms.